Höhler M., Alcázar-Román AR., Schenk K., Aguirre-Huamani MP., Braun C., Zrieq R., Mölleken K., Hegemann JH., Fleig U. Direct targeting of host microtubule and actin cytoskeletons by a chlamydial pathogenic effector protein. J Cell Sci. 2024 Sep 1; 137(17):jcs263450. https://doi.org/10.1242/jcs.263450  

Wevers C., Höhler M., Alcázar-Román AR., Hegemann JH., Fleig U. A Functional Yeast-Based Screen Identifies the Host Microtubule Cytoskeleton as a Target of Numerous Chlamydia pneumoniae Proteins. Int J Mol Sci. 2023 Apr 20;24(8):7618. https://doi.org/10.3390/ijms24087618

Kuenzel NA., Alcázar-Román AR., Saiardi A., Bartsch SM., Daunaraviciute S., Fiedler D., Fleig U.  Inositol pyrophosphate-controlled kinetochore architecture and mitotic entry in S. pombe. J. Fungi 2022, 8(9), 933. https://doi.org/10.3390/jof8090933

Pascual-Ortiz, M.; Walla, E.; Fleig, U.; Saiardi, A. The PPIP5K Family Member Asp1 Controls Inorganic Polyphosphate Metabolism in S. pombe. J. Fungi 2021, 7, 626. https://doi.org/10.3390/jof7080626

Rosenbach H, Walla E, Cutsail GE 3rd, Birrell JA, Pascual-Ortiz M, DeBeer S, Fleig U, Span I. The Asp1 pyrophosphatase from S. pombe hosts a [2Fe-2S]²⁺ cluster in vivo. (2021) Pubmed

Braun C, Alcázar-Román AR, Laska A, Mölleken K, Fleig U, Hegemann JH. CPn0572, the C. pneumoniae ortholog of Tarp, reorganizes the actin cytoskeleton via a newly identified F-actin binding domain and recruitment of vinculin. (2019) Pubmed

Riquelme M, Aguirre J, Bartnicki-García S, Braus GH, Feldbrügge M, Fleig U, Hansberg W, Herrera-Estrella A, Kämper J, Kück U, Mouriño-Pérez RR, Takeshita N, Fischer R. Fungal Morphogenesis, from the Polarized Growth of Hyphae to Complex Reproduction and Infection Structures. (2018) Pubmed

Pascual-Ortiz M, Saiardi A, Walla E, Jakopec V, Künzel NA, Span I, Vangala A, Fleig U. Asp1 bi-functional activity modulates spindle function via controlling cellular inositol pyrophosphate levels in Schizosaccharomyces pombe. (2018) Pubmed

Topolski B, Jakopec V, Künzel NA, Fleig U. The inositol pyrophosphate kinase Asp1 modulates chromosome segregation fidelity and spindle function in S.pombe. (2016) Pubmed

Pöhlmann J, Risse C, Seidel C, Pohlmann T, Jakopec V, Walla E, Ramrath P, Takeshita N, Baumann S, Feldbrügge M, Fischer R, Fleig U. The Vip 1 Inositol Polyphosphate Kinase Family Regulates Polarized Growth and Modulates the Microtubule Cytoskeleton in Fungi. PLoS Genet. (2014) Pubmed

Hegemann JH, Heick SB, Pöhlmann J, Langen MM, Fleig U. Targeted gene deletion in Saccharomyces cerevisiae and Schizosaccharomyces pombe. Methods Mol Biol. (2014) Pubmed

Kriegenburg F, Jakopec V, Poulsen EG, Nielsen SV, Roguev A, Krogan N, Gordon C, Fleig U, Hartmann-Petersen R. A chaperone-assisted degradation pathway targets kinetochore proteins to ensure genome stability. PLoS Genet. (2014) Pubmed

Jakopec V, Topolski B, Fleig U. Sos7, an essential component of the conserved Schizosaccharomyces pombe Ndc80-MIND-Spc7 complex, identifies a new family of fungal kinetochore proteins. Mol Cell Biol. (2012) Pubmed

Jakopec V, Walla E, Fleig U. Versatile use of Schizosaccharomyces pombe plasmids in Saccharomyces cerevisiae. FEMS Yeast Res. (2011) Pubmed

Pöhlmann J, Fleig U. Asp1, a conserved 1/3 insositol polyphosphate kinase, regulates the dimorphic switch in Schizosaccharomyces pombe. Mol Cell Biol. (2010) Pubmed

Kerres A, Jakopec V, Fleig U. The conserved Spc7 protein is required for spindle integrity and links kinetochore complexes in fission yeast. Mol Biol Cell. (2007) Pubmed

Grallert A, Beuter C, Craven RA, Bagley S, Wilks D, Fleig U, Hagan IM. S. pombe CLASP needs dynein, not EB1 or CLIP170, to induce microtubule instability and slows polymerization rates at cell tips in a dynein-dependent manner.Genes Dev. (2006) 20(17): 2421-36 Pubmed

Kerres A, Jakopec V, Beuter C, Karig I, Pohlmann J, Pidoux A, Allshire R, Fleig U. Fta2, an essential fission yeast kinetochore component, interacts closely with the conserved Mal2 protein. Mol Biol Cell. (2006) 17(10): 4167-7  Pubmed

Sanchez-Perez I, Renwick SJ, Crawley K, Karig I, Buck V, Franco-Sanchez A, Fleig U, Toda T, Millar JB. The DASH complex and Klp5/Klp6 kinesin coordinate bipolar chromosome attachment in fission yeast. EMBO J. (2005) 24(16): 2931-43  Pubmed

Kerres A, Vietmeier-Decker C, Ortiz J, Karig I, Beuter C, Hegemann JH, Lechner J, Fleig U. The Fission Yeast Kinetochore Component Spc7 Associates with the EB1 Family Member Mal3 and is required for Kinetochore-Spindle Association. Mol Biol Cell. (2004) 15(12): 5255-67 Pubmed

Jin QW, Pidoux A, Decker C, Allshire R and Fleig U. The Mal2p protein is an essential component of the fission yeast centromere. Mol Biol Cell. (2002) 22(20): 7168-83 Pubmed

Fiedler T, Karpova TS, Fleig U, Young ME, Cooper JA and Hegemann JH. The vesicular transport protein Cgp1p/Rki1p/Vps54p/Tcs3p/Luv1p is required for the integrity of the actin cytoskeleton. Mol Genet Genomics. (2002) 268(2): 190-205 Pubmed

Fleig U, Salus SS, Karig I and Sazer S. The fission yeast RAN GTPase is required for microtubule integrity. J. Cell Biol. (2000) 151: 1101-1111 Pubmed

Bechert T, Heck S, Fleig U, Diekmann S and Hegemann JH. All 16 centromere DNAs from Saccharomyces cerevisiae show DNA curvature. Nucleic Acid Res. (1999) 27: 1444-1449 Pubmed

Hegemann JH, Klein S, Güldener U, Niedenthal R and Fleig U. A fast method to diagnose chromosome and plasmid loss in Saccharomyces cerevisiae strains. Yeast (1999) 15: 1009-1019 Pubmed

Gould, K., Feoktistova, A. and Fleig U. A phosphorylation site mutant of Schizosaccharomyces pombe cdc2p fails to promote metaphase to anaphase transition. MGG (1998) 259: 437-448 Pubmed

Beinhauer, J., Hagan, I., Hegemann, J.H. and Fleig U. Mal3, the fission yeast homologue of the human APC-interacting protein EB1 is required for microtubule integrity and the maintenance of cell form. J. Cell Biol. (1997) 139: 717-728 Pubmed

Fleig U, Sen-Gupta M and Hegemann JH. Fission yeast mal2+ is required for chromosome segregation. Mol. Cell Biol. (1996) 16: 6169-6177 Pubmed

Sen-Gupta M, Lyck R, Fleig U, Niedenthal RK and Hegemann JH. The sequence of a 24152 bp segment from the left arm of chromosome XIV from Saccharomyces cerevisiae between the BNI1 and the POL2 genes. Yeast (1996) 12: 505-514  Pubmed

Fleig U, Beinhauer JD, Hegemann JH. Functional selection for the centromere DNA from yeast chromosome VIII. Nucleic Acids Res. (1995) 23(6):922-4 Pubmed

Hegemann JH, Fleig U.The centromere of budding yeast. Bioessays (1993) 15(7): 451-60 Review Pubmed

Fleig U, Gould KL, Nurse P. A dominant negative allele of p34cdc2 shows altered phosphoamino acid content and sequesters p56cdc13 cyclin. Mol Cell Biol. 1992 May;12(5):2295-301 Pubmed

Nitschke K, Fleig U, Schell J, Palme K. Complementation of the cs dis2-11 cell cycle mutant of Schizosaccharomyces pombe by a protein phophatase from Arabidopsis thaliana. EMBO J. 1992 Apr;11(4):1327-33 Pubmed

Fleig U, Gould KL. Complementation of the cs dis2-11 cell cycle mutant of Schizosaccharomyces pombe: the role of phosphorylation. Semin Cell Biol. 1991 Aug;2(4):195-204. Review Pubmed

Fleig U, Nurse P. Expression of a dominant negative allele of cdc2 prevents activation of the endogenous p34cdc2 kinase. Mol Gen Genet. 1991 May;226(3):432-40 Pubmed

Fleig U, Nurse P. Construction of LYS2 cartridges for use in genetic manipulation of Saccharomyces cerevisiae. Gene. 1986;46(2-3):237-45  Pubmed